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Macklin Inc nile red
Nile Red, supplied by Macklin Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nile+red/nile+red/pm42308948-40-7-12
Average 86 stars, based on 1 article reviews
nile red - by Bioz Stars, 2026-10
86/100 stars

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Article Title: Fabrication of dysphagia-friendly emulsion gels using tilapia protein: Synergistic effects of high-density ultrasonic modification and flaxseed gum interfacial complexation.
Article Snippet: Protein-based emulsion gels hold considerable potential for mitigating dysphagia in elderly populations owing to their tunable mesostructure and swallowing adaptability.. This study explored the feasibility of employing ultrasonicated tilapia protein (UTP) complexed with flaxseed gum (FG) as composite stabilizers to prepare emulsion gels for dysphagia individuals.. Results demonstrated that ultrasonication reduced the particle size of TP and modified its spatial conformation, thereby strengthening molecular interactions between UTP and FG and promoting the formation of stable complexes with improved solubility and emulsifying capacity.

Article Title: Optimization of LF-NMR-based methods for analysis of oil content and distribution in germinating oilseeds.
Article Snippet: BACKGROUND: Lipid metabolism is critical for seed germination, directly impacting their nutritional value as a food raw material.. Conventional methods for oil analysis are destructive and fail to determine oil distribution.. This study evaluated the feasibility of using low-field nuclear magnetic resonance (LF-NMR) coupled with magnetic resonance imaging (MRI) as a nondestructive approach for monitoring oil changes in germinating oilseeds.

Article Title: Optimizing steaming core temperature to enhance tilapia protein digestibility in an elderly model: structural and textural mechanisms.
Article Snippet: Trichloroacetic acid (TCA), analytical grade salts and Nile red were purchased from Macklin Biochemical & Technology Co., Ltd. (Shanghai, China).

Article Title: Lignocellulose nanofibrils from oat hulls stabilize plant-based milk via interfacial adsorption and network formation
Article Snippet: The inherent strong hydrophilicity of pure nanocellulose (CNF) limits their effectiveness as Pickering stabilizers due to insufficient adsorption energy at the oil-water interface.. Herein, we valorized oat hulls into lignocellulose nanofibrils (LCNF) through a low-concentration alkali treatment strategy that allowed for controlled lignin retention, thereby enhancing amphiphilicity and interfacial activity.. The resulting LCNF was characterized by excellent amphiphilicity, a high negative surface charge, good thermal stability, and nanoscale diameters (down to 26.72 nm), surpassing the performance of pure CNF.

Article Title: Interfacial and emulsifying properties of low-denatured soy protein prepared by self-coacervation
Article Snippet: The formation of denatured and aggregated proteins during conventional extensive wet extraction processes limits the functionality of plant proteins.. Although the effectively minimizing denaturation and preserving native protein structure by the mild extraction methods have shown promise in enhancing functional properties, the mechanisms underlying the performance of low-denatured plant proteins remain poorly understood.. Thus, investigating the interfacial behavior of low-denatured plant proteins is essential to unravel their structurefunction relationships and enable the targeted applications in multiphase food systems.

Article Title: Aqueous biphasic systems based on dual deep eutectic solvents for simultaneous extraction and targeted partitioning of bioactive compounds from Cinnamomum cassia.
Article Snippet: A green extraction method using dual deep eutectic solvents (DESs) in aqueous biphasic systems (ABSs) was developed for Cinnamomum cassia (C. cassia).. 16 DES-based ABSs were constructed utilizing IL-based DESs (I) as the chaotropic agent and DESs (II) as the kosmotropic agent.. An optimal system (DES I:DES II:H2O = 3:3:4) efficiently extracted polysaccharides, phenols, flavonoids, and organic acids.

Protease Inhibitor:

Article Title: Inhibition of Calcium‐Dependent Lipid Droplets Relocation of ACSL4‐PKCβ‐ALOX15 Complex Alleviates Ferroptosis and Acute Pancreatitis
Article Snippet: .. Discovery Probe‐FDA‐approved‐Drug‐Library (Cat: L1021, APExBIO), PKC412 (Midostaurin; Cat: HY‐10230, MCE), Ruboxistaurin ( LY333531 ; Cat: HY‐10195, MCE), EDTA‐AM (Cat: HY‐D1746, MCE), EGTA‐AM (Cat: HY‐D0973, MCE), BHQ (Cat: S3628, Selleckchem), Ferrostatin‐1(Fer‐1; Cat: HY‐100579, MCE), Deferoxamine (Cat: HY‐B1625 MCE), Erastin (Cat: HY‐15763, MCE), RSL3 (Cat: HY‐100218A, MCE), Xinidipine (Cat: 132203‐70‐4, MACKLIN), Benidipine (Cat: 105979‐17‐7, MACKLIN), Manidipine (Cat: 89226‐50‐6, MACKLIN), Lacidipine (Cat: 103890‐78‐4, MACKLIN), Nisoldipine (Cat: N858016, MACKLIN), Z‐VAD‐FMK (Cat: 161401‐82‐7, TargetMol), Lipi‐Blue (Cat: L247‐LD01, Dojindo), Liperfluo (Cat: NG661, Dojindo), BODIPYTM 493/503 (Cat: D3922, Invitrogen), Nile Red (Cat: 7385‐67‐3, MACKLIN), Lipofectamine 2000 (Cat: 11668‐019, Thermo Fisher), Anti‐fluorescence fade sealing reagent (Cat: S2100, Solarbio), PF‐04620110 (Cat: T6937, TargetMol), PF‐06424439 (Cat: T12425 , TargetMol), Ingenol‐5,20‐acetonide‐3‐O‐angelate (Cat: TQ0316, TargetMol), Protease Inhibitor Cocktail (Cat: K1007, APExBIO), Taq PCR Master Mix (Cat: K1034, APExBIO), RIPA lysate (Cat: PR20035, Proteintech), Mycoplasma Removal Agent Plus (Cat: C0290S, Beyotime), Penicillin‐Streptomycin (Cat: P1400, Solarbio), Trypsin‐EDTA (Cat: 25200072, Gibco), BayK8644 (Cat: 98625‐26‐4, MACKLIN), Raptinal (Cat: 1176‐09‐6, TargetMol), BODIPYTM 581/591 (Cat: 217075‐36‐0, TargetMol), TNF‐α (Cat: TMPY‐00936, TargetMol), propidium iodide (Cat: C0080, Solarbio), Smac mimetics (Cat: T2080, TargetMol), ML218 (Cat: T12076L, TargetMol), .. Pierce ECL Plus Western Blotting Substrate (Cat: 32209, Thermo Fisher Scientific), SYBR Green PCR Master Mix (Cat: A46109, Thermo Fisher), Prime Script RT reagent Kit with gDNA Eraser (Perfect Real Time; Cat: RR047A, Takara Bio), TaqMan Universal PCR Master Mix (Cat: 4304437, Thermo Fisher), Gene JET Gel Extraction Kit (Cat: K0691, Thermo Fisher), Axygen Prep DNA Gel Extraction Kit (Cat: AP‐MX‐P‐25, Axygen), TaqMan Reverse Transcription (Cat: N8080234, Thermo Fisher Scientific), Mycoplasma Removal Agent (Cat: C0288M, Beyotime), TIAN prep Mini Plasmid Kit (Cat: DP103‐03, TIANGEN), Enhanced BCA Protein Assay Kit (Cat: P0010, Beyotime), Lightening cloning Kit (Cat: BDIT0014, Biodragon), Protein A/G Magnetic Beads (Cat: HY‐K0202, MCE).

Polymerase Chain Reaction:

Article Title: Inhibition of Calcium‐Dependent Lipid Droplets Relocation of ACSL4‐PKCβ‐ALOX15 Complex Alleviates Ferroptosis and Acute Pancreatitis
Article Snippet: .. Discovery Probe‐FDA‐approved‐Drug‐Library (Cat: L1021, APExBIO), PKC412 (Midostaurin; Cat: HY‐10230, MCE), Ruboxistaurin ( LY333531 ; Cat: HY‐10195, MCE), EDTA‐AM (Cat: HY‐D1746, MCE), EGTA‐AM (Cat: HY‐D0973, MCE), BHQ (Cat: S3628, Selleckchem), Ferrostatin‐1(Fer‐1; Cat: HY‐100579, MCE), Deferoxamine (Cat: HY‐B1625 MCE), Erastin (Cat: HY‐15763, MCE), RSL3 (Cat: HY‐100218A, MCE), Xinidipine (Cat: 132203‐70‐4, MACKLIN), Benidipine (Cat: 105979‐17‐7, MACKLIN), Manidipine (Cat: 89226‐50‐6, MACKLIN), Lacidipine (Cat: 103890‐78‐4, MACKLIN), Nisoldipine (Cat: N858016, MACKLIN), Z‐VAD‐FMK (Cat: 161401‐82‐7, TargetMol), Lipi‐Blue (Cat: L247‐LD01, Dojindo), Liperfluo (Cat: NG661, Dojindo), BODIPYTM 493/503 (Cat: D3922, Invitrogen), Nile Red (Cat: 7385‐67‐3, MACKLIN), Lipofectamine 2000 (Cat: 11668‐019, Thermo Fisher), Anti‐fluorescence fade sealing reagent (Cat: S2100, Solarbio), PF‐04620110 (Cat: T6937, TargetMol), PF‐06424439 (Cat: T12425 , TargetMol), Ingenol‐5,20‐acetonide‐3‐O‐angelate (Cat: TQ0316, TargetMol), Protease Inhibitor Cocktail (Cat: K1007, APExBIO), Taq PCR Master Mix (Cat: K1034, APExBIO), RIPA lysate (Cat: PR20035, Proteintech), Mycoplasma Removal Agent Plus (Cat: C0290S, Beyotime), Penicillin‐Streptomycin (Cat: P1400, Solarbio), Trypsin‐EDTA (Cat: 25200072, Gibco), BayK8644 (Cat: 98625‐26‐4, MACKLIN), Raptinal (Cat: 1176‐09‐6, TargetMol), BODIPYTM 581/591 (Cat: 217075‐36‐0, TargetMol), TNF‐α (Cat: TMPY‐00936, TargetMol), propidium iodide (Cat: C0080, Solarbio), Smac mimetics (Cat: T2080, TargetMol), ML218 (Cat: T12076L, TargetMol), .. Pierce ECL Plus Western Blotting Substrate (Cat: 32209, Thermo Fisher Scientific), SYBR Green PCR Master Mix (Cat: A46109, Thermo Fisher), Prime Script RT reagent Kit with gDNA Eraser (Perfect Real Time; Cat: RR047A, Takara Bio), TaqMan Universal PCR Master Mix (Cat: 4304437, Thermo Fisher), Gene JET Gel Extraction Kit (Cat: K0691, Thermo Fisher), Axygen Prep DNA Gel Extraction Kit (Cat: AP‐MX‐P‐25, Axygen), TaqMan Reverse Transcription (Cat: N8080234, Thermo Fisher Scientific), Mycoplasma Removal Agent (Cat: C0288M, Beyotime), TIAN prep Mini Plasmid Kit (Cat: DP103‐03, TIANGEN), Enhanced BCA Protein Assay Kit (Cat: P0010, Beyotime), Lightening cloning Kit (Cat: BDIT0014, Biodragon), Protein A/G Magnetic Beads (Cat: HY‐K0202, MCE).



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Senescence-targeted, antibacterial, and hemostatic properties of composite hydrogels. (A) Fluorescence images of young and senescent PDLSCs (D-gal and LPS pretreated) incubated with Nile red (NR)-loaded <t>Gal-MPDA</t> for the indicated time periods. (B) Confocal fluorescence images showing the subcellular localization of GM@Nir in D-gal and LPS-induced senescent PDLSCs and cross-sectional analysis. Lysosome, Lyso tracker; Nuclei, Hoechst. (C) Live-dead staining for PDLSCs cells on day 1,3,5. (D) The viability of PDLSCs cells assessed by CCK-8 assay cocultured with composite hydrogels on day 1,3,5. (E) Representative optical images of the Hemolytic test after treating mouse blood with TA, TAM-GM, or TAM-GM@Met, followed by quantification analysis. (F) Live/dead staining of E. coli, S. aureus and P. gingivalis . ns, not significant. Data are presented as means ± SEM (n = 3 per subgroup). ∗∗∗P < 0.001.
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Image Search Results


Senescence-targeted, antibacterial, and hemostatic properties of composite hydrogels. (A) Fluorescence images of young and senescent PDLSCs (D-gal and LPS pretreated) incubated with Nile red (NR)-loaded Gal-MPDA for the indicated time periods. (B) Confocal fluorescence images showing the subcellular localization of GM@Nir in D-gal and LPS-induced senescent PDLSCs and cross-sectional analysis. Lysosome, Lyso tracker; Nuclei, Hoechst. (C) Live-dead staining for PDLSCs cells on day 1,3,5. (D) The viability of PDLSCs cells assessed by CCK-8 assay cocultured with composite hydrogels on day 1,3,5. (E) Representative optical images of the Hemolytic test after treating mouse blood with TA, TAM-GM, or TAM-GM@Met, followed by quantification analysis. (F) Live/dead staining of E. coli, S. aureus and P. gingivalis . ns, not significant. Data are presented as means ± SEM (n = 3 per subgroup). ∗∗∗P < 0.001.

Journal: Bioactive Materials

Article Title: Metformin alleviates aging-associated periodontitis via NRF2-mediated restoration of the IRE1α dependent unfolded protein response

doi: 10.1016/j.bioactmat.2026.03.060

Figure Lengend Snippet: Senescence-targeted, antibacterial, and hemostatic properties of composite hydrogels. (A) Fluorescence images of young and senescent PDLSCs (D-gal and LPS pretreated) incubated with Nile red (NR)-loaded Gal-MPDA for the indicated time periods. (B) Confocal fluorescence images showing the subcellular localization of GM@Nir in D-gal and LPS-induced senescent PDLSCs and cross-sectional analysis. Lysosome, Lyso tracker; Nuclei, Hoechst. (C) Live-dead staining for PDLSCs cells on day 1,3,5. (D) The viability of PDLSCs cells assessed by CCK-8 assay cocultured with composite hydrogels on day 1,3,5. (E) Representative optical images of the Hemolytic test after treating mouse blood with TA, TAM-GM, or TAM-GM@Met, followed by quantification analysis. (F) Live/dead staining of E. coli, S. aureus and P. gingivalis . ns, not significant. Data are presented as means ± SEM (n = 3 per subgroup). ∗∗∗P < 0.001.

Article Snippet: For fluorescence labeling, Nile red (HY-D0718, MCE) was added to 1 mg/mL MPDA nanoparticles and stirred at room temperature in the dark for 12 h. Then, galactan was added at a ratio of 1:2 with MPDA and stirred for another 12 h in the dark at room temperature.

Techniques: Fluorescence, Incubation, Staining, CCK-8 Assay

In vivo therapeutic effect of the TAM-GM@Met on mice with an aging-associated periodontitis model. (A) Schematic illustration of the therapeutic process on the D-gal induced aging mice periodontitis model. (B) Micro-CT reconstructions and buccal-palatal sectional views of maxillary molars and evaluation of the distance from the CEJ to the ABC. (C, D) Mice periodontal tissue sections prepared for H&E staining (C) and Masson's trichrome staining (D). (E) Schematic of TAM-GM@Met preparation. TAM hydrogel incorporates galactose-coated MPDA nanoparticles (GM) to target senescent cells for metformin delivery. Metformin activates NRF2, leading to transcriptional upregulation of IRE1α, restoration of the UPR, and suppression of SASP in PDLSCs. ns, not significant; TAM, tannic acid and Ag-MOFs based hydrogel; MPDA, mesoporous polydopamine; UPR, unfolded protein response. Data are presented as means ± SEM (n = 5 per subgroup). ∗∗∗P < 0.001.

Journal: Bioactive Materials

Article Title: Metformin alleviates aging-associated periodontitis via NRF2-mediated restoration of the IRE1α dependent unfolded protein response

doi: 10.1016/j.bioactmat.2026.03.060

Figure Lengend Snippet: In vivo therapeutic effect of the TAM-GM@Met on mice with an aging-associated periodontitis model. (A) Schematic illustration of the therapeutic process on the D-gal induced aging mice periodontitis model. (B) Micro-CT reconstructions and buccal-palatal sectional views of maxillary molars and evaluation of the distance from the CEJ to the ABC. (C, D) Mice periodontal tissue sections prepared for H&E staining (C) and Masson's trichrome staining (D). (E) Schematic of TAM-GM@Met preparation. TAM hydrogel incorporates galactose-coated MPDA nanoparticles (GM) to target senescent cells for metformin delivery. Metformin activates NRF2, leading to transcriptional upregulation of IRE1α, restoration of the UPR, and suppression of SASP in PDLSCs. ns, not significant; TAM, tannic acid and Ag-MOFs based hydrogel; MPDA, mesoporous polydopamine; UPR, unfolded protein response. Data are presented as means ± SEM (n = 5 per subgroup). ∗∗∗P < 0.001.

Article Snippet: For fluorescence labeling, Nile red (HY-D0718, MCE) was added to 1 mg/mL MPDA nanoparticles and stirred at room temperature in the dark for 12 h. Then, galactan was added at a ratio of 1:2 with MPDA and stirred for another 12 h in the dark at room temperature.

Techniques: In Vivo, Micro-CT, Staining